Melanoma Marker antibody (CF®594)

Catalog No. ABIN6157201

The Mouse Monoclonal anti-Melanoma Marker antibody has been validated for IHC and IF. It is suitable to detect Melanoma Marker in samples from Human, Dog and Mouse.

Quick Overview for Melanoma Marker antibody (CF®594) (ABIN6157201)

Target: Melanoma Marker

Reactivity: Human, Dog, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma Marker antibody is conjugated to CF®594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PNL2

Product Details anti-Melanoma Marker Antibody (CF®594)

Purpose: Mouse Monoclonal anti-Melanoma Marker (PNL2), CF594 Conjugate

Characteristics: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Melanocyte antigen

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Melanoma Marker

Target: Melanoma Marker

Background: Human Melanoma Associated Antigen, PNL2

Molecular Weight: Multiple (160 kDa, 100 kDa and <10 kDa)