VCAM1 antibody (CF®647)

Catalog No. ABIN6157679

This anti-VCAM1 antibody is a Mouse Monoclonal antibody detecting VCAM1 in FACS, IF and IHC (f). Suitable for Human.

Quick Overview for VCAM1 antibody (CF®647) (ABIN6157679)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 1-4C3

Product Details anti-VCAM1 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD106 / VCAM1 (1.4C3), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 110 kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human umbilical vein endothelial cells (HUVEC)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Human placenta or tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106 / VCAM1

Background: CD106, INCAM-100, L1CAM, Vascular Cell Adhesion Molecule 1 (VCAM1), Vascular cell adhesion protein 1

Molecular Weight: ~110 kDa

Gene ID: 7412, 109225

UniProt: P19320

Pathways: Carbohydrate Homeostasis