Thrombomodulin antibody (AA 69-194) (Biotin)

Catalog No. ABIN6157890

This Mouse Monoclonal antibody specifically detects Thrombomodulin in WB, IHC, ELISA, FACS, IF, Coat and ISt. It exhibits reactivity toward Human.

Quick Overview for Thrombomodulin antibody (AA 69-194) (Biotin) (ABIN6157890)

Target: Thrombomodulin (THBD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunostaining (ISt)

Clone: THBD-1591

Product Details anti-Thrombomodulin Antibody (Biotin)

Binding Specificity: AA 69-194

Purpose: Mouse Monoclonal anti-Thrombomodulin / CD141 (THBD/1591), Biotin Conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• ELISA 2-4 μg/mL for coating (order Ab without BSA)
•   pH 9.0
• for 10-20 min followed by cooling at RT for 20 min
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: Thrombomodulin / CD141

Background: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)

Molecular Weight: 100 kDa

Gene ID: 7056, 2030

UniProt: P07204