Thrombomodulin antibody (AA 69-194) (CF®488A)

Catalog No. ABIN6157921

The Mouse Monoclonal anti-Thrombomodulin antibody has been validated for IHC, ELISA, FACS, IF, Coat and ISt. It is suitable to detect Thrombomodulin in samples from Human.

Quick Overview for Thrombomodulin antibody (AA 69-194) (CF®488A) (ABIN6157921)

Target: Thrombomodulin (THBD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunostaining (ISt)

Clone: THBD-1782

Product Details anti-Thrombomodulin Antibody (CF®488A)

Binding Specificity: AA 69-194

Purpose: Mouse Monoclonal anti-Thrombomodulin / CD141 (THBD/1782), CF488A Conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: Thrombomodulin / CD141

Background: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)

Molecular Weight: 100 kDa

Gene ID: 7056, 2030

UniProt: P07204