Nuclear Membrane antibody (Biotin)
Quick Overview for Nuclear Membrane antibody (Biotin) (ABIN6159638)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Mouse Monoclonal anti-Nuclear Membrane (AE-5), Biotin Conjugate
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Characteristics
- This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Immunogen
- Nuclei of myeloid leukemia biopsy cells
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Isotype
- IgG1 kappa
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Application Notes
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Immunocytochemistry acetone-fixed cells 0.5-1.0 μg/mL
- Immunofluorescence 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
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Comment
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Tonsil
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS/0.1 % BSA/0.05 % azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Nuclear Membrane
Target
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