CD7 antibody (CF®568)

Catalog No. ABIN6160231

The Mouse Monoclonal anti-CD7 antibody has been validated for FACS and IF. It is suitable to detect CD7 in samples from Human, Guinea Pig and Pig.

Quick Overview for CD7 antibody (CF®568) (ABIN6160231)

Target: CD7

Reactivity: Human, Guinea Pig, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD7 antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B-F12

Product Details anti-CD7 Antibody (CF®568)

Purpose: Mouse Monoclonal anti-CD7 (B-F12), CF568 Conjugate

Characteristics: This antibody recognizes a protein of 40 kDa, identified as CD7 (also known as gp40, Leu9). CD7 is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Chronic Lymphoid Leukemia cells (CLL)

Isotype: IgG2a kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat, HUT-78, Molt-4, CEM cells, or human peripheral blood lymphocytes. Lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD7

Target: CD7

Alternative Name: CD7

Background: GP40, Leu9, p41, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41

Molecular Weight: 40 kDa

Gene ID: 924, 186820

UniProt: P09564

Pathways: Cell-Cell Junction Organization