Iba1 antibody (FITC)

Catalog No. ABIN616071

The FITC-conjugated Mouse Monoclonal anti-Iba1 antibody (Clone 1022-5) (ABIN616071) specifically detects Iba1 in FACS and IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for Iba1 antibody (FITC) (ABIN616071)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1022-5

Product Details anti-Iba1 Antibody (FITC)

Specificity: The antibody reacts with AIF-1.

Cross-Reactivity (Details): Species reactivity (tested):Human (Activated Macrophages) and Rat.

Purification: Affinity Chromatography

Immunogen: Purified Human recombinant AIF-1. Epitope not further characterized.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 10 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Storage: 4 °C/-20 °C

Storage Comment: Store the lyophilized antibody at 2-8 °C. Freeze stock solution and aliquots thereof at -20 °C.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activated macrophages and microglial cells. It has been suggested that AIF-1 expression is associated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 and MRP14, is expressed by activated macrophages and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. The recently described apoptosis-inducing factor AIF is not related to AIF-1.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gene ID: 199

NCBI Accession: NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration