VWF antibody (AA 845-949) (CF®640R)

Catalog No. ABIN6161513

This anti-VWF antibody is a Mouse Monoclonal antibody detecting VWF in WB, IHC, IP, FACS and IF. Suitable for Human.

Quick Overview for VWF antibody (AA 845-949) (CF®640R) (ABIN6161513)

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 3E2D10

Product Details anti-VWF Antibody (CF®640R)

Binding Specificity: AA 845-949

Purpose: Mouse Monoclonal anti-von Willebrand Factor (3E2D10), CF640R Conjugate

Characteristics: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human vWF fragment spanning aa 845-949

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1.0 μg/mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Optimal dilution for a specific application should be determined by user

Comment:

HUVEC cells or Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: von Willebrand Factor

Background: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Molecular Weight: 250 kDa

Gene ID: 7450, 440848

UniProt: P04275