Cyclin B1 antibody (CF®488A)

Catalog No. ABIN6161645

This anti-Cyclin B1 antibody is a Mouse Monoclonal antibody detecting Cyclin B1 in IF, FACS and IHC (f). Suitable for Human and Mouse.

Quick Overview for Cyclin B1 antibody (CF®488A) (ABIN6161645)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CCNB1-1098

Product Details anti-Cyclin B1 Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-CyclinB1 (CCNB1/1098), CF488A Conjugate

Characteristics: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CCNB1 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CyclinB1

Background: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Molecular Weight: 55-62 kDa

Gene ID: 891, 23960

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase