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KRT1 antibody (CF®647)

KRT1 Reactivity: Human, Rat, Mouse, Rabbit, Chicken, Cow, Monkey, Dog WB, IF, FACS, IHC (f) Host: Mouse Monoclonal AE-3 CF®647
Catalog No. ABIN6161973
  • Target See all KRT1 Antibodies
    KRT1 (Cytokeratin HMW (KRT1))
    Reactivity
    • 27
    • 27
    • 18
    • 9
    • 9
    • 9
    • 9
    • 8
    • 1
    Human, Rat, Mouse, Rabbit, Chicken, Cow, Monkey, Dog
    Host
    • 27
    Mouse
    Clonality
    • 27
    Monoclonal
    Conjugate
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    This KRT1 antibody is conjugated to CF®647
    Application
    • 26
    • 26
    • 17
    • 15
    • 9
    • 9
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-Cytokeratin, HMW (AE-3), CF647 Conjugate
    Characteristics
    This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Human epidermal keratin
    Clone
    AE-3
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product KRT1 Primary Antibody
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Epithelial cells, Skin or Adenocarcinomas

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    KRT1 (Cytokeratin HMW (KRT1))
    Alternative Name
    Cytokeratin, HMW (KRT1 Products)
    Synonyms
    CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, keratin 1 antibody, KRT1 antibody
    Background
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
    Molecular Weight
    52-67 kDa
    Gene ID
    51350, 654392
    UniProt
    Q01546
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