FOXA1 antibody (AA 467-614) (CF®640R)

Catalog No. ABIN6163147

The Mouse Monoclonal anti-FOXA1 antibody has been validated for WB, IHC, FACS, IF and ISt. It is suitable to detect FOXA1 in samples from Human and Rat.

Quick Overview for FOXA1 antibody (AA 467-614) (CF®640R) (ABIN6163147)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXA1 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt)

Clone: FOXA1-1241

Product Details anti-FOXA1 Antibody (CF®640R)

Binding Specificity: AA 467-614

Purpose: Mouse Monoclonal anti-FOXA1 / HNF3A (FOXA1/1241), CF640R Conjugate

Characteristics: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (? and ?), HNF-3 (?, ? and ?), HNF-4 (? and ?), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FOXA1 protein fragment (aa467-614) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: FOXA1 / HNF3A

Background: Forkhead box protein A1 (FOXA1), Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A), Transcription factor 3A (TCF-3A or TCF3A)

Molecular Weight: 79 kDa

Gene ID: 3169, 163484

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis