HLA-A antibody (CF®488A)

Catalog No. ABIN6164077

The Mouse Monoclonal anti-HLA-A antibody has been validated for FACS and IF. It is suitable to detect HLA-A in samples from Human.

Quick Overview for HLA-A antibody (CF®488A) (ABIN6164077)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-A antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 108-2C5

Product Details anti-HLA-A Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-HLA-A (MHC-I) (108-2C5), CF488A Conjugate

Characteristics: HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the i±1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the i±1 domain was demonstrated in the formation of an intralocus HLA-A determinant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Normal human peripheral blood lymphocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

THP-1 and Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Alternative Name: HLA-A (MHC-I)

Background: HLA-A, HLA class 1A, MHC class I HLA-A

Molecular Weight: ~41 kDa

Gene ID: 3105, 181244, 654404

UniProt: P01889, P30443, P30499

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response