JUP antibody (CF®647)

Catalog No. ABIN6164937

This Mouse Monoclonal antibody specifically detects JUP in WB, IF, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for JUP antibody (CF®647) (ABIN6164937)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: CTNG-1483

Product Details anti-JUP Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Catenin, gamma (CTNG/1483), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 80-87 kDa, identified as gamma-catenin. The catenins (?, ?, ? and ?) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. ? T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. ? T-catenin binds to ? E-catenin as well as to ?-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for ?-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length gamma catenin protein

Isotype: IgG1 kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunohistology (formalin) 0.1-0.2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431, T47D or MCF-7 cells. Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Catenin, gamma

Background: ARVD12, Catenin (cadherin-associated protein), gamma 80  kDa, Catenin gamma, CTNNG, Desmoplakin III, Desmoplakin-3, DP3, DPIII, Junction Plakoglobin, PDGB, PKGB

Molecular Weight: 80-87 kDa

Gene ID: 3728, 514174

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location