LHCGR antibody (N-Term) (CF®488A)

Catalog No. ABIN6165103

The Mouse Monoclonal anti-LHCGR antibody has been validated for IF, FACS and IHC. It is suitable to detect LHCGR in samples from Human.

Quick Overview for LHCGR antibody (N-Term) (CF®488A) (ABIN6165103)

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LHCGR antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: LHCGR-1417

Product Details anti-LHCGR Antibody (CF®488A)

Binding Specificity: N-Term

Purpose: Mouse Monoclonal anti-Luteinizing Hormone / Choriogonadotropin Receptor (LHCGR/1417), CF488A Conjugate

Characteristics: This antibody recognizes a protein of 78-85 kDa, which is identified as luteinizing hormone/choriogonadotropin receptor. Luteinizing hormone plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Choriogonadotropin production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor (LHCGR) as a seven transmembrane, G protein-coupled receptor glycoprotein. LHCGR influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of LHCGR on breast carcinoma correlates in part to the degree of tumor differentiation. LHCGR -positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant N-terminal fragment of human LHCGR (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 or Jurkat cells. Ovarian Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for LHCGR

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Alternative Name: Luteinizing Hormone / Choriogonadotropin Receptor

Molecular Weight: 78-85 kDa

Gene ID: 3973, 468490

UniProt: P22888

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, cAMP Metabolic Process, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling