Nucleolin antibody

Catalog No. ABIN6166144

The Mouse Monoclonal anti-Nucleolin antibody has been validated for WB, IHC, IF, FACS and ISt. It is suitable to detect Nucleolin in samples from Human.

Quick Overview for Nucleolin antibody (ABIN6166144)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Clone: NCL-902

Product Details anti-Nucleolin Antibody

Purpose: Mouse Monoclonal anti-Nucleolin (NCL/902), Purified, BSA-free

Characteristics: This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human NCL protein

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with mouse, rat or cow, others not known

• Immunohistology (formalin)
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin s lymphoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: NCL, Nucl, Nucleolin, Protein C23

Molecular Weight: 76 kDa

Gene ID: 4691, 79110

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization