Fibronectin antibody (CF®647)

Catalog No. ABIN6168682

The Mouse Monoclonal anti-Fibronectin antibody has been validated for IF, FACS and IHC (f). It is suitable to detect Fibronectin in samples from Human, Rat, Mouse and Pig.

Quick Overview for Fibronectin antibody (CF®647) (ABIN6168682)

Target: Fibronectin

Reactivity: Human, Rat, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is conjugated to CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 2755-8

Product Details anti-Fibronectin Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Fibronectin (2755-8), CF647 Conjugate

Characteristics: Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: T-cell lymphoma biopsy

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

SW156 cells or Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Fibronectin

Target: Fibronectin

Background: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

Molecular Weight: 220 kDa (monomer), 440 kDa (dimer)

Gene ID: 2335, 203717

UniProt: P02751