CD45RB antibody (CF®488A)

Catalog No. ABIN6170558

This anti-CD45RB antibody is a Mouse Monoclonal antibody detecting CD45RB in FACS, IHC and IF. Suitable for Human.

Quick Overview for CD45RB antibody (CF®488A) (ABIN6170558)

Target: CD45RB

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: DF-B1

Product Details anti-CD45RB Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-CD45RB (DF-B1), CF488A Conjugate

Characteristics: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Isotype: IgG3 kappa

Application Details

Application Notes: Does not react with rat, others not known

• Immunohistology (formalin)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Ramos, U-698, or GA-10 cells. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB

Background: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Molecular Weight: 180-220 kDa

Gene ID: 5788, 654514

UniProt: P08575