Keratin 6 antibody (C-Term) (CF®594)

Catalog No. ABIN6170978

This anti-Keratin 6 antibody is a Mouse Monoclonal antibody detecting Keratin 6 in IF, FACS and IHC (f). Suitable for Human and Mouse.

Quick Overview for Keratin 6 antibody (C-Term) (CF®594) (ABIN6170978)

Target: Keratin 6 (KRT6)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6 antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LHK6

Product Details anti-Keratin 6 Antibody (CF®594)

Binding Specificity: C-Term

Purpose: Mouse Monoclonal anti-Cytokeratin 6 (LHK6), CF594 Conjugate

Characteristics: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Tonsil or basal cell carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Keratin 6

Target: Keratin 6 (KRT6)

Alternative Name: Cytokeratin 6

Molecular Weight: 56 kDa

Gene ID: 700779