Desmin antibody (CF®594)

Catalog No. ABIN6171206

This anti-Desmin antibody is a Mouse Monoclonal antibody detecting Desmin in WB, IHC, IF and FACS. Suitable for Human, Rat, Mouse, Chicken and Hamster.

Quick Overview for Desmin antibody (CF®594) (ABIN6171206)

Target: Desmin (DES)

Reactivity: Human, Rat, Mouse, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: DES-1711

Product Details anti-Desmin Antibody (CF®594)

Purpose: Mouse Monoclonal anti-Desmin (DES/1711), CF594 Conjugate

Characteristics: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain ?-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. The antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length desmin protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

SJRH30 Cells. Uterus or Leiomyosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background: CMD1I, CSM1, CSM2, DES, Intermediate filament protein

Molecular Weight: 52 kDa

Gene ID: 1674, 594952

UniProt: P17661