TGFA antibody (AA 34-43) (CF®488A)

Catalog No. ABIN6171432

This Mouse Monoclonal antibody specifically detects TGFA in IHC, IF and FACS. It exhibits reactivity toward Human, Rabbit and Zebrafish (Danio rerio).

Quick Overview for TGFA antibody (AA 34-43) (CF®488A) (ABIN6171432)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rabbit, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: P-T1

Product Details anti-TGFA Antibody (CF®488A)

Binding Specificity: AA 34-43

Purpose: Mouse Monoclonal anti-TGF alpha (P/T1), CF488A Conjugate

Characteristics: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A 10-amino acid synthetic peptide (aa34-43, PPVAAAVVSH) from human TGF?.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-paraffin 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat or Ramos cells. Testicular Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF alpha

Background: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Molecular Weight: 6 kDa

Gene ID: 7039, 170009

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway