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FLI1 antibody (AA 107-261) (CF®488A)

The Mouse Monoclonal anti-FLI1 antibody has been validated for WB, IF and FACS. It is suitable to detect FLI1 in samples from Human.
Catalog No. ABIN6172648

Quick Overview for FLI1 antibody (AA 107-261) (CF®488A) (ABIN6172648)

Target

See all FLI1 Antibodies
FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This FLI1 antibody is conjugated to CF®488A

Application

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

FLI1-1312
  • Binding Specificity

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    AA 107-261

    Purpose

    Mouse Monoclonal anti-FLI1 (FLI1/1312), CF488A Conjugate

    Characteristics

    This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Recombinant fragment (aa 107-261) of human FLI1 protein (exact sequence is proprietary)

    Isotype

    IgG
  • Application Notes

    Western blotting 1-2 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    Comment

    THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light
  • Target

    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

    Alternative Name

    FLI1

    Molecular Weight

    51 kDa

    Gene ID

    2313, 504281

    UniProt

    Q01543
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