FLI1 antibody (AA 107-261) (CF®488A)
Quick Overview for FLI1 antibody (AA 107-261) (CF®488A) (ABIN6172648)
Target
See all FLI1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 107-261
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Purpose
- Mouse Monoclonal anti-FLI1 (FLI1/1312), CF488A Conjugate
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Characteristics
- This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Immunogen
- Recombinant fragment (aa 107-261) of human FLI1 protein (exact sequence is proprietary)
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Isotype
- IgG
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Application Notes
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Western blotting 1-2 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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Comment
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THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS/0.1 % BSA/0.05 % azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Protect from light
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- FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
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Alternative Name
- FLI1
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Molecular Weight
- 51 kDa
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Gene ID
- 2313, 504281
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UniProt
- Q01543
Target
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