NEFH antibody (Biotin)

Catalog No. ABIN6172769

This anti-NEFH antibody is a Mouse Monoclonal antibody detecting NEFH in FACS and IHC (f). Suitable for Human, Rat, Mouse, Chicken and Pig.

Quick Overview for NEFH antibody (Biotin) (ABIN6172769)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse, Chicken, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NF421

Product Details anti-NEFH Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Neurofilament (NF-H) (NF421), Biotin Conjugate

Characteristics: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human recombinant neurofilament protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Brain, Neuroblastoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament (NF-H)

Background: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200  kDa, Neurofilament Triplet H Protein, NF-H, NF200

Molecular Weight: 200 kDa

Gene ID: 4744, 198760

UniProt: P12036