EPO antibody (AA 28-162) (Biotin)

Catalog No. ABIN6174289

The Mouse Monoclonal anti-EPO antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect EPO in samples from Human.

Quick Overview for EPO antibody (AA 28-162) (Biotin) (ABIN6174289)

Target: EPO (Erythropoietin (EPO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPO antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: EPO-1368

Product Details anti-EPO Antibody (Biotin)

Binding Specificity: AA 28-162

Purpose: Mouse Monoclonal anti-Erythropoietin (EPO/1368), Biotin Conjugate

Characteristics: This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human EPO protein (aa28-162) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: Immunohistology (formalin) 4-8 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 Cells. Heart or Kidney.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: Erythropoietin

Target Type: Hormone

Background: EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2

Molecular Weight: 37 kDa

Gene ID: 2056, 2303

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity