Cytokeratin 18 antibody (CF®640R)

Catalog No. ABIN6179166

This Mouse Monoclonal antibody specifically detects Cytokeratin 18 in IF, FACS and IHC (f). It exhibits reactivity toward Human, Mouse, Rat, Dog, Pig, Cat, Cow, Hamster, Sheep, Horse, Ferret, Goat, Marmoset and Monkey.

Quick Overview for Cytokeratin 18 antibody (CF®640R) (ABIN6179166)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cat, Cow, Hamster, Sheep, Horse, Ferret, Goat, Marmoset, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is conjugated to CF®640R

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C-04

Product Details anti-Cytokeratin 18 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-Cytokeratin 18 (C-04), CF640R Conjugate

Characteristics: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with chicken or Xenopus, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7, HeLa cells, Breast Cancer

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18

Background: Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

Molecular Weight: 45 kDa

Gene ID: 3875, 406013

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis