HTR2C antibody (AA 439-460)

Catalog No. ABIN617931

This anti-HTR2C antibody is a Rabbit Polyclonal antibody detecting HTR2C in WB, IHC, IF, ICC and IEM. Suitable for Mouse and Rat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for HTR2C antibody (AA 439-460) (ABIN617931)

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR2C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Product Details anti-HTR2C Antibody

Binding Specificity: AA 439-460

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Characteristics: The 5-HT 2C Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor coupled to KLH and bovine thyroglobulin. The 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.

Purification: Affinity Purified

Immunogen: Rat 5-HT2C receptor (439-460)

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References for anti-HTR2C antibody (ABIN617931)

Zhang, Li, Xin, Lao, Ren, Berman, Tan, Zhang: "Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model." in: Neurochemical research, Vol. 36, Issue 10, pp. 1785-92, (2011) (PubMed).

Coulon, Kanyshkova, Broicher, Munsch, Wettschureck, Seidenbecher, Meuth, Offermanns, Pape, Budde: "Activity Modes in Thalamocortical Relay Neurons are Modulated by G(q)/G(11) Family G-proteins - Serotonergic and Glutamatergic Signaling." in: Frontiers in cellular neuroscience, Vol. 4, pp. 132, (2011) (PubMed).

Yang, Mahaffey, Bérubé, Maddatu, Cox, Frankel: "Complex seizure disorder caused by Brunol4 deficiency in mice." in: PLoS genetics, Vol. 3, Issue 7, pp. e124, (2007) (PubMed).

Holmes: "5-Hydroxytryptamine2C receptors on pudendal motoneurons innervating the external anal sphincter." in: Brain research, Vol. 1057, Issue 1-2, pp. 65-71, (2005) (PubMed).

Target Details for HTR2C

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Alternative Name: Serotonin Receptor 2C (HTR2C)

Background: Other Names:
5-hydroxytryptamine receptor 2C,5-HT2C, 5HT-1C, 5-HTR2C,5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled

Gene ID: 25187

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour