SOX2 antibody (AA 176-305) (Biotin)

Catalog No. ABIN6180445

The Mouse Monoclonal anti-SOX2 antibody has been validated for WB, ELISA, IHC, IF, FACS and Coat. It is suitable to detect SOX2 in samples from Human.

Quick Overview for SOX2 antibody (AA 176-305) (Biotin) (ABIN6180445)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: SOX2-1791

Product Details anti-SOX2 Antibody (Biotin)

Binding Specificity: AA 176-305

Purpose: Mouse Monoclonal anti-SOX2 (SOX2/1791), Biotin Conjugate

Characteristics: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SOX2 protein fragment (aa176-305) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 cells or Cervical carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2

Background: ANOP3, Delta EF2a, MCOPS3 (Microphthalmia Syndromic type 3), SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb

Molecular Weight: 34 kDa

Gene ID: 6657, 518438

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis