SOX10 antibody (AA 115-269) (CF®568)

Catalog No. ABIN6181114

The Mouse Monoclonal anti-SOX10 antibody has been validated for WB, FACS and IF. It is suitable to detect SOX10 in samples from Human.

Quick Overview for SOX10 antibody (AA 115-269) (CF®568) (ABIN6181114)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX10 antibody is conjugated to CF®568

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SOX10-992

Product Details anti-SOX10 Antibody (CF®568)

Binding Specificity: AA 115-269

Purpose: Mouse Monoclonal anti-SOX10 (SOX10/992), CF568 Conjugate

Characteristics: This antibody recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 cells. Melanomas, breast carcinomas, gliomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background: DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E (WS2E), WS4, Waardenburg syndrome type 4C (WS4C)

Molecular Weight: 49-58 kDa

Gene ID: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding