Nkx2-2 antibody (CF®488A)

Catalog No. ABIN6181502

This anti-Nkx2-2 antibody is a Mouse Monoclonal antibody detecting Nkx2-2 in IF, FACS and IHC (f). Suitable for Human, Mouse, Rat and Chicken.

Quick Overview for Nkx2-2 antibody (CF®488A) (ABIN6181502)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NX2-294

Product Details anti-Nkx2-2 Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-NKX2.2 (NX2/294), CF488A Conjugate

Characteristics: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human full-length recombinant NKX2.2 protein

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Pancreas or Ewing s sarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman

Molecular Weight: 40-50 kDa

Gene ID: 4821, 516922

UniProt: O95096

Pathways: Dopaminergic Neurogenesis