MUC1 antibody (CF®405S)

Catalog No. ABIN6184102

The Mouse Monoclonal anti-MUC1 antibody has been validated for ELISA, FACS, IF, IHC (f) and Coat. It is suitable to detect MUC1 in samples from Human and Mouse.

Quick Overview for MUC1 antibody (CF®405S) (ABIN6184102)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is conjugated to CF®405S

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: 139H2

Product Details anti-MUC1 Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (139H2), CF405S Conjugate

Characteristics: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human milk-fat globule membranes (HMFGM)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1-2 μg/mL
• ELISA For coating use Ab at 2-5 μg/mL, order Ab without BSA
• Flow Cytometry 0.25-0.5 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: Mucin 1 / EMA / Episialin / CD227

Molecular Weight: 265-400 kDa

Gene ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling