Clathrin Heavy Chain (CLTC) (N-Term) antibody (CF®647)

Catalog No. ABIN6185554

The Mouse Monoclonal anti- antibody has been validated for WB, IF and FACS. It is suitable to detect in samples from Human.

Quick Overview for Clathrin Heavy Chain (CLTC) (N-Term) antibody (CF®647) (ABIN6185554)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CLTC-1431

Product Details

Binding Specificity: N-Term

Purpose: Mouse Monoclonal anti-Clathrin, Heavy chain (CLTC/1431), CF647 Conjugate

Characteristics: This antibody recognizes protein of 192 kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Heavy Chain (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa cells. Placenta or Prostate Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin, Heavy chain

Background: Clathrin heavy chain, Clathrin heavy chain 1, Clathrin heavy chain 2, Clathrin heavy chain on chromosome 17 (CHC17 or CHC-17), CLH22, CLTC, CLTCL, CLTCL1, CLTCL2, CLTD

Molecular Weight: 192 kDa

Gene ID: 1213, 491351