FBXO5 antibody (AA 1-250) (CF®640R)

Catalog No. ABIN6185968

The Mouse Monoclonal anti-FBXO5 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect FBXO5 in samples from Human.

Quick Overview for FBXO5 antibody (AA 1-250) (CF®640R) (ABIN6185968)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBXO5 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: EMI1-1176

Product Details anti-FBXO5 Antibody (CF®640R)

Binding Specificity: AA 1-250

Purpose: Mouse Monoclonal anti-EMI1 (EMI1/1176), CF640R Conjugate

Characteristics: This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 1-2 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: EMI1

Background: Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31

Molecular Weight: 56 kDa

Gene ID: 26271, 520506

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases