GM-CSF antibody (CF®488A)

Catalog No. ABIN6186100

The Rat Monoclonal anti-GM-CSF antibody has been validated for WB, IP, FACS, Neut, IF and IHC (fro). It is suitable to detect GM-CSF in samples from Human, Cynomolgus and Rhesus Monkey.

Quick Overview for GM-CSF antibody (CF®488A) (ABIN6186100)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to CF®488A

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Neutralization (Neut), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BVD2-21C11

Product Details anti-GM-CSF Antibody (CF®488A)

Purpose: Rat Monoclonal anti-GM-CSF (BVD2-21C11), CF488A Conjugate

Characteristics: Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GM-CSF protein

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Neutralization Studies order Ab without azide
• Optimal dilution for a specific application should be determined by user

Comment:

Lymph node and tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Molecular Weight: 22 kDa

Gene ID: 1437, 1349

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin