MUC2 antibody (CF®568)

Catalog No. ABIN6186700

The Mouse Monoclonal anti-MUC2 antibody has been validated for IF, FACS and IHC (f). It is suitable to detect MUC2 in samples from Human.

Quick Overview for MUC2 antibody (CF®568) (ABIN6186700)

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC2 antibody is conjugated to CF®568

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CCP58

Product Details anti-MUC2 Antibody (CF®568)

Purpose: Mouse Monoclonal anti-MUC2 (CCP58), CF568 Conjugate

Characteristics: This antibody recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon, in about 65 % of colonic carcinomas and about 40 % of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

LS174T cells or small intestine.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Alternative Name: MUC2

Background: Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC

Molecular Weight: 520 kDa

Gene ID: 4583, 315

UniProt: Q02817