MYOD1 antibody (MYD712) (CF®594)

Catalog No. ABIN6188420

This anti-MYOD1 antibody is a Mouse Monoclonal antibody detecting MYOD1 in IF, FACS, IHC (f) and Diff. Suitable for Human, Mouse, Rat and Chicken.

Quick Overview for MYOD1 antibody (MYD712) (CF®594) (ABIN6188420)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Differentiation (Diff)

Clone: 5-8A MYD712

Product Details anti-MYOD1 Antibody (CF®594)

Binding Specificity: MYD712

Purpose: Mouse Monoclonal anti-MyoD1 (5.8A + MYD712), CF594 Conjugate

Characteristics: This antibody recognizes a phosphor-protein of 45 kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant mouse MyoD1 protein (5.8A), Recombinant human MyoD1 protein (MYD712)

Isotype: IgG

Application Details

Application Notes: Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Rhabdomyosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MyoD1

Background: BHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Molecular Weight: 45 kDa

Gene ID: 4654, 181768

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development