GRP78 antibody

Catalog No. ABIN618936

This anti-GRP78 antibody is a Chicken Polyclonal antibody detecting GRP78 in WB and IF. Suitable for Arabidopsis thaliana, Spinach, Zea mays, Moss and Barley.

Quick Overview for GRP78 antibody (ABIN618936)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Arabidopsis thaliana, Spinach, Zea mays, Moss, Barley

Host: Chicken

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-GRP78 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including: Nicotiana tabacum, Spinacia oleracea, monocots: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens

Characteristics: Expected / apparent Molecular Weight of the Antigene: 73.5 / 80 kDa

Purification: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3

Application Details

Application Notes: 1: 2000 with standard ECL (WB), 1: 50 to 1: 1000 (IF)

Comment:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Restrictions: For Research Use only

Handling

Buffer: PBS pH 7.4, liquid

Storage: 4 °C

Storage Comment: store at 4°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: BiP

Background: AGI Code: At1g09080, At1g42020
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

Molecular Weight: expected: 73.5 kDa, apparent: 80 kDa

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling