TNFSF15 antibody (CF®640R)

Catalog No. ABIN6189578

The CF®640R-conjugated Mouse Monoclonal anti-TNFSF15 antibody (Clone VEGI-1283) (ABIN6189578) specifically detects TNFSF15 in WB, IHC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for TNFSF15 antibody (CF®640R) (ABIN6189578)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF15 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: VEGI-1283

Product Details anti-TNFSF15 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-VEGI (VEGI/1283), CF640R Conjugate

Characteristics: VEGI is an anti-angiogenic cytokine that belongs to tumor necrosis factor superfamily, member 15 (TNFSF15). This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Reduced expression of VEGI has been reported as a marker of poor prognosis in breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length VEGI fragment

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 1-2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Endothelial cells. Colon, intestine, placenta, lung, liver, kidney, pancreas, spleen and prostate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for TNFSF15

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: VEGI

Background: TNF lig,-related molecule 1 (TL1A), Tumor necrosis factor (lig,) superfamily member 15 (TNFSF15), Vascular endothelial growth inhibitor 192a, Vascular endothelial growth inhibitor (VEGI), VEGI192A

Molecular Weight: 22 kDa

Gene ID: 9966, 241382

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy