Testosterone antibody

Catalog No. ABIN6189877

Product Details anti-Testosterone Antibody

Target: Testosterone

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Testosterone antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Immunostaining (ISt)

Purpose: Mouse Monoclonal anti-Testosterone (4E1G2), Purified, BSA-free

Characteristics: This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~10¹⁰ M^-1. In competitive binding immunoassay, it reacts with testosterone 100 % , 11-beta-hydroxy testosterone 3.3 %, 17-alpha-methyl testosterone <0.1 %, 5-alpha DHT 0.8 %, estradiol <0.1 % and progesterone <0.1 %. Testosterone, a steroid hormone from the androgen group, is derived from cholesterol. Testosterone is primarily secreted in the testes, but the ovaries and adrenal glands also produce smaller amounts. It is the principal male sex hormone and is responsible for the maturation of sex organs and the development of male secondary sex characteristics. The hormone is also involved in the growth of muscle mass and increases bone density and maturation. It functions by activating the androgen receptor and converting to estradiol and activation of estrogen receptors. Testosterone has been employed therapeutically to treat many conditions including infertility, lack of libido, osteoporosis, height growth and bone marrow stimulation. Anti-testosterone antibody may also prove useful in identification of testicular tumors arising from interstitial cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Testosterone 3 CMO conjugated to BSA

Clone: 4E1G2

Isotype: IgG1 kappa

Application Details

Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0 for 10-20 min followed by cooling at RT for 20 min

• Immunohistology formalin-paraffin 0.25-0.5 μg/mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Comment:

Interstitial or Leydid cells in testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for Testosterone

Target: Testosterone

Abstract: Testosterone Products

Target Type: Hormone

Background: 17i²-Hydroxy-3-oxo-4-androstene, 17i²-Hydroxy-4-androsten-3-one, 4-Androsten-17i²-ol-3-one, trans-Testosterone

Molecular Weight: 288.42Da