Recombinant CA 19-9 antibody (Biotin)

Catalog No. ABIN6190553

This Rabbit Monoclonal antibody specifically detects CA 19-9 in IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Recombinant CA 19-9 antibody (Biotin) (ABIN6190553)

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CA 19-9 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: CA19-9-1390R

Product Details anti-CA 19-9 Antibody (Biotin)

Purpose: Recombinant Rabbit Monoclonal anti-CA19-9/Sialyl-Lewisa (CA19.9/1390R), Biotin Conjugate

Characteristics: CA19-9, a carbohydrate epitope expressed on a high MW (>400 kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human CA19-9 protein

Isotype: IgG kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL Immunohistology (formalin) 5-10 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer  pH 6.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Stomach or Colon Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CA 19-9

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Alternative Name: CA19-9/Sialyl-Lewisa

Background: CA19.9, Ovarian tumor antigen, Pancreatic tumor antigen, Sialyl Lewis a

Molecular Weight: >400 kDa