ACTH antibody (N-Term) (CF®647)

Catalog No. ABIN6190912

This anti-ACTH antibody is a Mouse Monoclonal antibody detecting ACTH in IHC, IF, FACS, ELISA and Coat. Suitable for Human, Rat and Mouse.

Quick Overview for ACTH antibody (N-Term) (CF®647) (ABIN6190912)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Coating (Coat)

Clone: 57

Product Details anti-ACTH Antibody (CF®647)

Binding Specificity: N-Term

Purpose: Mouse Monoclonal anti-ACTH (57), CF647 Conjugate

Characteristics: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: N-terminal fragment of human ACTH conjugated to KLH

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• ELISA For coating, order Ab without BSA
• Predicted to show broad species reactivity
• Optimal dilution for a specific application should be determined by user

Comment:

Normal pituitary gland or pituitary tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH (Adrenocorticotrophic Hormone)

Target Type: Hormone

Background: Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC

Molecular Weight: ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Gene ID: 5443, 1897

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity