Elastase 3B antibody (AA 82-238) (CF®647)

Catalog No. ABIN6191520

The Mouse Monoclonal anti-Elastase 3B antibody has been validated for WB, IHC, ELISA, Coat, IF and FACS. It is suitable to detect Elastase 3B in samples from Human.

Quick Overview for Elastase 3B antibody (AA 82-238) (CF®647) (ABIN6191520)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CELA3B-1757

Product Details anti-Elastase 3B Antibody (CF®647)

Binding Specificity: AA 82-238

Purpose: Mouse Monoclonal anti-CELA3B / ELA3B (CELA3B/1757), CF647 Conjugate

Characteristics: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Panc-28 or PANC1 cells. Pancreas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: CELA3B / ELA3B

Background: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E

Molecular Weight: 17 kDa

Gene ID: 23436, 728752

UniProt: P08861