AGO6 antibody
Quick Overview for AGO6 antibody (ABIN619533)
Target
Reactivity
Host
Clonality
Application
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Purpose
- Anti-AGO6 | Argonaute 6
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Cross-Reactivity (Details)
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Arabidopsis thaliana
Not reactive in: Poplar sp., Zea mays -
Predicted Reactivity
- Arabidopsis thaliana
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Purification
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
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Application Notes
- 1 : 1000 (WB)
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Comment
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antibody binds microRNA and tasiRNAs, preference for 24nt siRNAs with 5'UAGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- AGO6 (Argonaute 6 (AGO6))
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Alternative Name
- AGO6
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Background
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Background: AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Additional information: There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6 % denaturing gel to obtain good separation.If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.
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Molecular Weight
- 116.4 | 99 kDa
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Gene ID
- 817856
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UniProt
- O48771
Target
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