AGO6 antibody

Catalog No. ABIN619533

This anti-AGO6 antibody is a Rabbit Polyclonal antibody detecting AGO6 in WB. Suitable for Arabidopsis thaliana.

Quick Overview for AGO6 antibody (ABIN619533)

Target: AGO6 (Argonaute 6 (AGO6))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-AGO6 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 116.4 / 99

Purification: serum

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940

Application Details

Application Notes: 1: 1000 (WB)

Comment:

antibody binds microRNA and tasiRNAs, preference for 24nt siRNAs with 5'UAGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μg of sterile water.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for AGO6

Target: AGO6 (Argonaute 6 (AGO6))

Alternative Name: AGO6

Background: AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molecular Weight: expected: 116.4 kDa, apparent: 99

UniProt: O48771