HSP18.5 antibody

Catalog No. ABIN619791

This anti-HSP18.5 antibody is a Rabbit Polyclonal antibody detecting HSP18.5 in WB. Suitable for Arabidopsis thaliana.

Quick Overview for HSP18.5 antibody (ABIN619791)

Target: HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-HSP18.5 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays

Characteristics: Expected / apparent Molecular Weight of the Antigene: 18.5 kDa

Purification: serum

Immunogen: recombinant Arabidopsis thaliana Hsp18.5

Application Details

Application Notes: 1 : 1000 with standard ECL (WB)

Comment:

hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for HSP18.5

Target: HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))

Alternative Name: HSP18.5

Background: HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein

Molecular Weight: 18.5 kDa