Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HSP18.5 antibody

HSP18.5-C Reactivity: Arabidopsis thaliana WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN619791
$634.95
Plus shipping costs $45.00
200 μL
Shipping to: United States
Delivery in 6 to 8 Business Days
  • Target
    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
    Reactivity
    Arabidopsis thaliana
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity are currently known
    Predicted Reactivity
    dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 18.5 kDa
    Purification
    serum
    Immunogen
    recombinant Arabidopsis thaliana Hsp18.5
  • Application Notes
    1 : 1000 with standard ECL (WB)
    Comment

    hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 μL of sterile water.
    Storage
    -20 °C
    Storage Comment
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
    Alternative Name
    HSP18.5
    Background
    HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
    Molecular Weight
    18.5 kDa
You are here:
Support