DMPO antibody

Catalog No. ABIN619793

The Mouse Monoclonal anti-DMPO antibody is suitable to detect DMPO. It has been validated for IP, ELISA, WB, ICC and IST.

Quick Overview for DMPO antibody (ABIN619793)

Target: DMPO

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immuno-Spin Trapping (IST)

Clone: N1664A

Product Details

Purpose: Anti-DMPO | DMPO nitrone adduct (clone N1664A)

Cross-Reactivity (Details): DMPO (species independent)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: DMPO (species independent)

Purification: Protein G purified

Immunogen: 5

Isotype: IgG1

Application Details

Application Notes: 1-10 μg/mL (ELISA), 10 μg/mL (ICC), 25 μg (IP), 1-10 μg/mL (WB)

Comment:

Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Total IgG. Protein G purified.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Expiry Date: 12 months

Target Details

Target: DMPO

Target Type: Chemical

Background:

Background: DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Additional information: Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.

Molecular Weight: 90 kDa