EED antibody (AA 269-303)

Catalog No. ABIN6242307

The Rabbit Polyclonal anti-EED antibody has been validated for WB and IHC (p). It is suitable to detect EED in samples from Human and Mouse.

Quick Overview for EED antibody (AA 269-303) (ABIN6242307)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB51180

Product Details anti-EED Antibody

Binding Specificity: AA 269-303

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This mouse Eed antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 269-303 amino acids from the Central region of mouse Eed.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: Eed

Background: Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.

Molecular Weight: 50198

UniProt: Q921E6