RNF2 antibody (AA 165-199)

Catalog No. ABIN6242607

The Rabbit Polyclonal anti-RNF2 antibody has been validated for WB. It is suitable to detect RNF2 in samples from Human and Mouse.

Quick Overview for RNF2 antibody (AA 165-199) (ABIN6242607)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB52402

Product Details anti-RNF2 Antibody

Binding Specificity: AA 165-199

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This mouse Rnf2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 165-199 amino acids from the Central region of mouse Rnf2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: Rnf2

Background: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Association to the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes.

Molecular Weight: 37623

UniProt: Q9CQJ4