Sonic Hedgehog antibody (C-Term)

Catalog No. ABIN6242829

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB and FACS. It is suitable to detect Sonic Hedgehog in samples from Mouse.

Quick Overview for Sonic Hedgehog antibody (C-Term) (ABIN6242829)

Target: Sonic Hedgehog (SHH)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: RB51214

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 397-431, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of mouse Shh.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).

Molecular Weight: 47773

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development