MAP1LC3A antibody (AA 30-56)

Catalog No. ABIN6243032

This Rabbit Polyclonal antibody specifically detects MAP1LC3A in WB, IF and IHC (p). It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MAP1LC3A antibody (AA 30-56) (ABIN6243032)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB11840

Product Details anti-MAP1LC3A Antibody

Binding Specificity: AA 30-56

Predicted Reactivity: Zf, B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from human LC3.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MAP1LC3A antibody (ABIN6243032)

Lu, Hsu: "Ambient temperature reduction extends lifespan via activating cellular degradation activity in an annual fish (Nothobranchius rachovii)." in: Age (Dordrecht, Netherlands), Vol. 37, Issue 2, pp. 33, (2015) (PubMed).

Hsu, Chuang, Chan: "Changes in cellular degradation activity in young and old worker honeybees (Apis mellifera)." in: Experimental gerontology, Vol. 50, pp. 128-36, (2014) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

NCBI Accession: NP_115903, NP_852610

UniProt: Q9H492, Q9GZQ8

Pathways: Autophagy