SUFUH antibody

Catalog No. ABIN6244107

The Mouse Monoclonal anti-SUFUH antibody has been validated for WB and IHC (p). It is suitable to detect SUFUH in samples from Human, Mouse and Green Monkey.

Quick Overview for SUFUH antibody (ABIN6244107)

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Reactivity: Human, Mouse, Green Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUFUH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1783CT536-263-29

Product Details anti-SUFUH Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This SUFU antibody is generated from a mouse immunized with a recombinant protein of human SUFU.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for SUFUH

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Alternative Name: SUFU

Background: Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU- GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity).

Molecular Weight: 53947

UniProt: Q9UMX1

Pathways: Hedgehog Signaling, Tube Formation, Maintenance of Protein Location