HCG alpha antibody

Catalog No. ABIN6251828

The Mouse Monoclonal anti-HCG alpha antibody (Clone CGA91-2) (ABIN6251828) specifically detects HCG alpha in IHC. The antibody is reactive with Human samples.

Quick Overview for HCG alpha antibody (ABIN6251828)

Target: HCG alpha

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CGA91-2

Product Details

Purification: Protein G purified monoclonal antibody

Immunogen: Recombinant protein was used as the immunogen for this HCG-alpha antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Variations in protocols, secondaries and substrates may require the HCG-alpha antibody to be titered for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Target Details

Target: HCG alpha

Alternative Name: HCG-alpha

Background: This antibody reacts with a protein of ~13  kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Gene ID: 1081

UniProt: P01215